TABLE 2.
Challenge phage mutations that decrease NAC binding
| Mutation(s) | No. iso- lateda | DNA sequenceb | In vitro DNA bindingc |
|---|---|---|---|
| Wild type | GGGCTCATAG GGTATTTGTA TGCGTGAGTT | + | |
| 5A | 2 | GGGCACATAG GGTATTTGTA TGCGTGAGTT | ± |
| 6T | 3 | GGGCTTATAG GGTATTTGTA TGCGTGAGTT | ± |
| 6G | 2 | GGGCTGATAG GGTATTTGTA TGCGTGAGTT | ± |
| 7G | 1 | GGGCTCGTAG GGTATTTGTA TGCGTGAGTT | ND |
| 7T | 1 | GGGCTCTTAG GGTATTTGTA TGCGTGAGTT | − |
| 8C | 6 | GGGCTCACAG GGTATTTGTA TGCGTGAGTT | − |
| 8A | 10 | GGGCTCAAAG GGTATTTGTA TGCGTGAGTT | − |
| 8G | 1 | GGGCTCAGAG GGTATTTGTA TGCGTGAGTT | − |
| 9G | 8 | GGGCTCATGG GGTATTTGTA TGCGTGAGTT | − |
| 11A | 2 | GGGCTCATAG AGTATTTGTA TGCGTGAGTT | ± |
| 12T | 1 | GGGCTCATAG GTTATTTGTA TGCGTGAGTT | ± |
| 14G | 2 | GGGCTCATAG GGTGTTTGTA TGCGTGAGTT | ND |
| 15C | 2 | GGGCTCATAG GGTACTTGTA TGCGTGAGTT | ± |
| 16G | 1 | GGGCTCATAG GGTATGTGTA TGCGTGAGTT | ± |
| 19C | 1 | GGGCTCATAG GGTATTTGCA TGCGTGAGTT | ND |
| 19A | 2 | GGGCTCATAG GGTATTTGAA TGCGTGAGTT | ± |
| 20T | 4 | GGGCTCATAG GGTATTTGTT TGCGTGAGTT | − |
| 20G | 2 | GGGCTCATAG GGTATTTGTG TGCGTGAGTT | ND |
| 21G | 1 | GGGCTCATAG GGTATTTGTA GGCGTGAGTT | − |
| 21A | 2 | GGGCTCATAG GGTATTTGTA AGCGTGAGTT | ± |
| 35C | 3 | GGGCTCATAG GGTATTTGTA TGCGCGAGTT | ± |
| 12Δ | 1 | GGGCTCATAG G-TATTTGTA TGCGTGAGTT | − |
| 13Δ | 1 | GGGCTCATAG GG-ATTTGTA TGCGTGAGTT | ND |
| 19Δ | 1 | GGGCTCATAG GGTATTTG-A TGCGTGAGTT | − |
| 8C, 9Δ | 1 | GGGCTCAC-G GGTATTTGTA TGCGTGAGTT | ND |
| 9Δ, 14T | 1 | GGGCTCAT-G GGTTTTTGTA TGCGTGAGTT | − |
| 13A, 14G, 15T | 1 | GGGCTCATAG GGAGTTTTGT ATGCGTGAGTT | ND |
| 19A, 20T | 1 | GGGCTCATAG GGTATTTGAT TGCGTGAGTT | ND |
| 19G, 20T | 1 | GGGCTCATAG GGTATTTGGT TGCGTGAGTT | ND |
a
Number of independent mutants with the same nucleotide changes.
b
Nucleotide changes are shown in bold and underlined.
c
In vitro binding indicates Relative binding affinity of NAC based upon gel retardation assays as shown in Figure 4. +, efficient binding; ±, weak binding; −, no binding detected. ND, not determined.