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. 2023 Dec 1;13:21174. doi: 10.1038/s41598-023-48373-1

Table 1.

Factors and levels of response surface analysis.

Variables Unit Symbol Variables level
Low −1 Center 0 High  + 1
MB initial concentration mg/L X1 50 100 150
BRB addition amount G X2 0.05 0.1 0.15
Reaction time min X3 30 90 150
Reaction temperature °C X4 20 30 40
pH X5 6 8 10