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. 2023 Jul 11;13(12):4840–4855. doi: 10.1016/j.apsb.2023.07.006

Figure 6.

Figure 6

CircCDK17 inhibited the proliferation and cell cycle progression of PASMCs. (A) Western blotting analysis showing that overexpression (OE) of circCDK17 attenuated hypoxia-stimulated PCNA expression, n = 6. (B–C) EdU staining (B) and CCK8 (C) analysis showing that OE-circCDK17 attenuated hypoxia-induced cell proliferation and viability, n = 6. (D) Flow cytometry showing the effect of OE-circCDK17 on cell cycle, n = 6. (E) RNA pull-down combined mass spectrometry showing circCDK17 binds with PCNA specific peptide. (F) Effect of circCDK17 overexpression on PCNA ubiquitination level, n = 3. (G) Western blotting analysis showing that silencing of circCDK17 attenuated the effect of ADAR1 gene knocking-down on protein expression of PCNA, Cyclin A and CDK1, n = 6. (H–I) CCK8 analysis (H) and EdU staining (I) showing that silencing of circCDK17 abolished the effect of ADAR1 deficiency on cell viability and proliferation in the presence of hypoxia, n = 6. NOR, normoxia; HYP, hypoxia; NC, negative control; SI, siRNA; OE, overexpression of circCDK17; Statistical analysis was performed with two-way ANOVA followed by Dunnett's test; All values are presented as mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.