Skip to main content
. 2023 Apr 18;13(12):5121–5134. doi: 10.1016/j.apsb.2023.04.006

Figure 6.

Figure 6

In vivo biodistribution and cancer targeting ability of DN@OEV in orthotopic SKOV3-Luc bearing ovarian cancer nude mouse. (A) In vivo fluorescence images of orthotopic SKOV3-Luc bearing ovarian cancer nude mouse at different time points after intraperitoneal injection of free Cy7, Cy7-DN and Cy7-DN@OEV. After 96 h, mice were sacrificed and their major organs and tumors were collected to detect Cy7 and Luc signals. (B) The corresponding semiquantitative results of ex vivo organs and tumors in different groups. (C) Confocal images of the tumor tissues in orthotopic ovarian cancer mouse after intraperitoneal injection of PBS, DOX, DN and DN@OEV. Scale bar: 50 μm. (D) Protocol of Zombie mouse and live mouse to verify the transcytosis effect DN@OEV in vivo. After development of Zombie mouse bearing orthotopic ovarian cancer, mouse was first fixed by PFA and circulated with DN@OEV. (E) DOX, DN and DN@OEV accumulation in tumor tissues of Zombie mouse and live mouse. Results are represented as mean ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001.