TABLE 1.
Transcriptional activation and binding in gdhA-lacZ chimeric promoters by NAC
| Clone | Nucleotide at positiona:
|
β-Gal sp act (U/mg of protein)b
|
Activity ratioc | Relative bindingd | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | +N | −N | |||
| 803 | A | T | A | A | G | G | G | G | A | A | C | T | G | A | T | 53 ± 38 | 54 ± 29 | 1.0 | 1.0 |
| 830 | A | T | A | A | c | a | a | a | A | t | t | g | t | A | T | 93 ± 35 | 2,048 ± 782 | 22 | 1.2 |
| 804 | A | T | A | A | G | G | G | G | A | A | C | T | t | A | T | 46 ± 21 | 46 ± 17 | 1.0 | 1.3 |
| 805 | A | T | A | A | c | a | a | a | A | A | C | T | G | A | T | 80 ± 22 | 100 ± 20 | 1.3 | 1.4 |
| 806 | A | T | A | A | c | a | a | a | A | A | C | T | t | A | T | 54 ± 26 | 106 ± 56 | 2.0 | 1.6 |
| 829 | A | T | A | A | G | G | G | G | A | t | t | g | G | A | T | 49 ± 10 | 124 ± 62 | 2.5 | 0.2 |
| 850 | A | T | A | A | G | G | G | G | A | A | C | g | t | A | T | 44 ± 3 | 303 ± 18 | 6.9 | 1.3 |
| 950 | A | T | A | A | c | a | G | G | A | A | C | g | t | A | T | 82 ± 30 | 355 ± 53 | 4.3 | 1.0 |
| 951 | A | T | A | A | G | G | G | G | A | t | C | g | t | A | T | 55 ± 5 | 807 ± 287 | 15 | 1.3 |
a
Altered nucleotides in the 15-bp core of the NAC-binding site are shown as lowercase letters.
b
Values are means ± standard deviations from three independent experiments. β-Gal, β-galactosidase.
c
Ratio of the activated (−N) to the basal (+N) value.
d
Percentage of shifted counts of each mutant normalized to the percentage of shifted counts in the wild type (pCB803).