TABLE 2.
Transcriptional activation and binding in hutU-lacZ chimeric promoters by NAC
| Clone | Nucleotide at positiona:
|
β-Gal sp act (U/mg of protein)b
|
Activity ratioc | Relative bindingd | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | +N | −N | |||
| 648 | A | T | A | A | C | A | A | A | A | T | T | G | T | A | T | 58 ± 24 | 1,297 ± 137 | 22 | 1.0 |
| 807 | A | T | A | A | C | A | A | A | A | T | T | G | g | A | T | 37 ± 18 | 58 ± 12 | 1.6 | 0.1 |
| 808 | A | T | A | A | g | g | g | g | A | T | T | G | T | A | T | 52 ± 16 | 305 ± 81 | 5.9 | 0.4 |
| 831 | A | T | A | A | C | A | A | A | A | a | c | t | T | A | T | 59 ± 36 | 208 ± 34 | 3.5 | 2.0 |
| 853 | A | T | A | c | a | A | t | t | t | T | g | t | T | A | T | 95 ± 15 | 172 ± 81 | 1.8 | 0.7 |
| 899 | A | T | A | c | a | A | A | A | A | T | T | G | T | A | T | 44 ± 9 | 328 ± 186 | 7.5 | 0.2 |
| 902 | A | T | A | c | C | A | A | A | A | T | T | G | T | A | T | 67 ± 19 | 287 ± 122 | 4.3 | 0.2 |
| 901 | A | T | A | A | C | A | A | A | A | T | T | t | T | A | T | 57 ± 15 | 143 ± 26 | 2.5 | 0.6 |
| 900 | A | T | A | A | C | A | A | A | A | T | g | t | T | A | T | 65 ± 35 | 536 ± 320 | 8.2 | 1.6 |
| 903 | t | a | t | g | t | t | A | A | A | a | c | a | a | t | a | 42 ± 2 | 51 ± 5 | 1.2 | 0.1 |
| 952 | A | T | A | A | C | A | A | c | A | T | T | G | T | A | T | 57 ± 27 | 437 ± 112 | 7.7 | 0.2 |
| 953 | A | T | A | A | C | A | A | A | A | c | T | G | T | A | T | 53 ± 25 | 856 ± 276 | 16 | 0.4 |
a
Altered nucleotides in the 15-bp core of the NAC-binding site are shown as lowercase letters.
b
Values are means ± standard deviations from three independent experiments. β-Gal, β-galactosidase.
c
Ratio of the activated (−N) to the basal (+N) value.
d
Percentage of shifted counts of each mutant normalized to the percentage of shifted counts in the wild type (pCB648).