TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype and/or phenotype | Source (reference) |
|---|---|---|
| X. campestris | ||
| X59 (ATCC 55298) | B1459S-4L Rifr Gum+ | These authors (33) |
| m31 | X59 GumD− | These authors (33) |
| Sphingomonas | ||
| S88 | Strr Sps+ | ATCC 31554 (17) |
| m265 and m260 | S88 SpsB− | These authors (27) |
| m302 | S88 SpsB−K− | These authors (37) |
| R. leguminosarum bv. trifolii | ||
| RBL5515 | LPR5 cured of Sym plasmid, Strr Rifr | C. A. Wijffelman (28) |
| RBL5523 | RBL5515 pRL1JI spc-3::Tn1831 Pss+ | C. A. Wijffelman (25) |
| RBL5807 | RBL5523 Strr RifrpssA4::Tn5 (exo4) | C. A. Wijffelman (35) |
| RBL5833 | RBL5523 Strr RifrpssD111::Tn5 (exo111) | C. A. Wijffelman (35) |
| E. coli K-12 | ||
| DH5α | F− φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 relA1 supE44 hsdR17(rK− mK+)Δ(lacZYA-argF)U169 | Bethesda Research Laboratories |
| HMS174 | F−hsdR19 (rK− mK+) recA1 rpoB331 (Rifr)IN(rrnD-rrnE)1 | W. Studier |
| Plasmids | ||
| pRK2013 | Ori(ColE1) Kanr OriT(Mob+) Tra+ | D. Helinski (9) |
| pRK311 | OriV(RK2) Tetr OriT(Mob+) Tra− λcos lacZ(α) | D. Helinski (6) |
| pSEB24 | OriV(RSF1010) Camr Ampr OriT(Mob+) Tra− | These authors (37) |
| pMP92 | OriV(RK2) Tetr OriT(Mob+) Tra− | C. A. Wijffelman (32) |
| pMP2642 | pssA (RBL5515 DNA in pMP92) | These authors (35) |
| pMP3030 | pssCDE (RBL5515 DNA in pMP92) | These authors (35) |
| pXCc8 | gumBCDEFGHIJKLMN (X59 DNA in pRK311) | These authors (33) |
| pXC1483 | gumDEF (subclone of pXCc8) | These authors (33) |
| pS88c1Δ3 | spsDCEB rhsACBD (S88 DNA in pRK311) | These authors (37) |
| pS88c2 | spsGSRQIKLJFDCEB (S88 DNA in pRK311) | These authors (37) |
| pS88c3 | spsQIKLJFDCEB rhsACBD (S88 DNA in pRK311) | These authors (37) |
| pZ206 | pS88c2-spsQ::mini-Tn10kan | These authors (37) |
| pY976 | pS88c3-spsI::mini-Tn10kan | These authors (37) |
| pY882 | pS88c3-spsK::mini-Tn10kan | These authors (37) |
| pY872 | pS88c3-spsL::mini-Tn10kan | These authors (37) |
| pB215 | spsB (subclone of pS88c3 in pSEB24) | These authors (37) |
| pB608 | spsK (S88 DNA in pMP92) | This work |
| pB554 | spsKB (S88 DNA in pMP92) | This work |
| pB610 | gumKLM SpsB (X59 and S88 DNA in pMP92) | This work |
| pB611 | gumKLM (X59 DNA in pMP92) | This work |
| pB597 | pssCDE spsB (S88 DNA and pMP3030 subclone in pMP92 | This work |
| pB599 | pssDE spsB (S88 DNA and pMP3030 subclone in pMP92) | This work |
| pB609 | pssCD spsB (S88 DNA and pMP3030 subclone in pMP92) | This work |