TABLE 2.
C. jejuni promoter fragments cloned in pMW10
| Transformant | Insert sizea (bp) | Accession no. | β-Galactosidase activity (Miller units) ± SD in:
|
Primer used for cDNA synthesis | Distance of RNA start site to 3′-end inserta (bp) | Homologyc | |
|---|---|---|---|---|---|---|---|
| C. jejuni | E. coli | ||||||
| 1B7 | 684 | AJ002416 | 80 ± 14 | 2 ± 2 | 1B7 | 162 | yabC |
| 1G9 | 1,000b | AJ002417 | 260 ± 41 | 2 ± 3 | 1G9 | 251 | |
| 2A12 | 1,400b | AJ002421 | 143 ± 9 | 4 ± 2 | UP | 53 | |
| 3D8 | 445 | AJ002419 | 103 ± 10 | 1,985 ± 263 | UP | 17 | |
| 4C7 | 666 | U38524 | 169 ± 17 | 9 ± 3 | 4C7 | 369 | |
| 4C11 | 574 | AJ002418 | 70 ± 9 | 363 ± 52 | 4C11 | 251 | icd |
| 5G10 | 489 | Z36940 | 210 ± 35 | 3 ± 3 | 5G10 | 156 | orf11 |
| 11B4 | 800b | AJ002422 | 116 ± 32 | 781 ± 55 | UP | 126 | |
| 12G7 | 432 | AJ002027 | 260 ± 24 | 46 ± 11 | 12G7 | 256 | glu-tRNA |
| 14B7 | 446 | AJ002420 | 59 ± 10 | 3,545 ± 731 | UP | 70 | |
| 23E5 | 900b | AJ002415 | 252 ± 27 | 427 ± 67 | 23E5 | 367 | metK |
| pMW10 | AJ001494 | 4 ± 1 | 3 ± 1 | ||||
The precise lengths were determined by sequencing.
The fragment size was estimated by digesting the promoter clones with EcoRI and examining the digest after electrophoresis in a 1% agarose gel.
Gene whose product is homologous to the product of the ORF of the corresponding transformant.