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. 1998 Feb;180(3):594–599. doi: 10.1128/jb.180.3.594-599.1998

TABLE 2.

C. jejuni promoter fragments cloned in pMW10

Transformant Insert sizea (bp) Accession no. β-Galactosidase activity (Miller units) ± SD in:
Primer used for cDNA synthesis Distance of RNA start site to 3′-end inserta (bp) Homologyc
C. jejuni E. coli
1B7 684 AJ002416 80 ± 14 2 ± 2 1B7 162 yabC
1G9 1,000b AJ002417 260 ± 41 2 ± 3 1G9 251
2A12 1,400b AJ002421 143 ± 9 4 ± 2 UP 53
3D8 445 AJ002419 103 ± 10 1,985 ± 263 UP 17
4C7 666 U38524 169 ± 17 9 ± 3 4C7 369
4C11 574 AJ002418 70 ± 9 363 ± 52 4C11 251 icd
5G10 489 Z36940 210 ± 35 3 ± 3 5G10 156 orf11
11B4 800b AJ002422 116 ± 32 781 ± 55 UP 126
12G7 432 AJ002027 260 ± 24 46 ± 11 12G7 256 glu-tRNA
14B7 446 AJ002420 59 ± 10 3,545 ± 731 UP 70
23E5 900b AJ002415 252 ± 27 427 ± 67 23E5 367 metK
pMW10 AJ001494 4 ± 1 3 ± 1
a

The precise lengths were determined by sequencing. 

b

The fragment size was estimated by digesting the promoter clones with EcoRI and examining the digest after electrophoresis in a 1% agarose gel. 

c

Gene whose product is homologous to the product of the ORF of the corresponding transformant.