Table 2.

Replicates and corresponding microenvironment analyzed for the three microbial species. Microenvironments were identified using the grid cells illustrated in Fig. 3. For each replicate and microenvironment, the three microbial species presented different numbers of spots and tracks detected. (*) in E. aerogenes microenvironment (Proximal to V) of Replicate 2 indicates that the considered grid cells include part of the underlying E3 and E4 cells; (*) in E. aerogenes microenvironment (Proximal to V) of Replicate 3 indicates that the considered grid cells include part of the above A1 cell.

Replicate	Microbial species	Microenvironment	Frames	Grid cells analyzed	Number of Spots	Number of Tracks
Replicate 1	P. parasitica	Proximal to KCI	[1–143]	A1-A2-A3	4248	231
		Distant from KCI	[1–143]	C1-C2-C3	3839	505
	V. microstoma	Sessile forms	[1–114]	A5-A6	114	1
		Free forms	[1–114]	C4-C5-C6	52	8
	E. aerogenes	Proximal to V	[1–81]	A5-A6	3626	661
		Distant from V	[1–81]	C3-C4	2234	332
Replicate 2	P. parasitica	Proximal to KCI	[1–143]	A1-A2	2758	166
		Distant from KCI	[1–143]	C3-C4	5123	480
	V. microstoma	Sessile forms	[1–43]	A4-A5	86	2
		Free forms	[1–43]	A4-A5	47	5
	E. aerogenes	Proximal to V	[1–143]	D3-D4*	10209	1580
		Distant from V	[1–143]	B1-B2	11742	1060
Replicate 3	P. parasitica	Proximal to KCI	[1–41]	A1-A2	552	56
		Distant from KCI	[1–41]	D2-D3	1044	179
	V. microstoma	Sessile forms	[1–25]	A1-A2	51	3
		Free forms	[1–25]	B5-C5	16	5
	E. aerogenes	Proximal to V	[1–143]	A1*	8727	1981
		Distant from V	[1–143]	B4-B5	6316	1196