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. 2023 Nov 7;26(12):108401. doi: 10.1016/j.isci.2023.108401

Figure 7.

Figure 7

Immunohistochemical detection of apoptotis in metastatic omentum and in mesothelial cells from ascites

Matched pairs of omental tissue and ascites were retrieved from n = 6 HGSC patients including one patient (OC_67) with early micrometastatic lesions which is depicted in the following. (A-E) Paraffin sections from omentum specimens with early metastasis. (A) Sections subjected to HE staining. (B) Sections stained for caspase 3 to detect apoptotic cells (asterisks). (C) Tumor cells identified by staining for cytokeratin MNF 116. (D) Mesothelial cells were not detectable by calretinin staining, most likely due to early desquamation of apoptotic mesothelium. (E) Staining for αSMA was applied to identify the caspase 3+ apoptotic cells as fibroblasts. (F–K) Paraffin sections of ascites cells stained for caspase 3 (F–H) and calretinin (I–K). Apoptotic mesothelial cells (arrows) were identified as caspase 3+/calretinin+ cells with typical mesothelial morphology. Scale bar, 100 μm in all panels. Magnification: 200x in (A–E) and 400x in (F–K).