Figure 2.

Evaluation of the effect of individual and dual knock down of c-MET and EphA2 genes on MD-MB-231 in the presence of HGF

(A) c-MET and EphA2 knockdown/inhibition and its effect on downstream effectors on MDA-MB-231 cells was analyzed by western blot; NTP = non-target pool control.

(B) Transwell migration assay post knockdown/inhibition of c-MET or EphA2 or both and HGF as a chemotactic factor on MDA-MB-231 cells.

(C) Transwell invasion assay post knockdown/inhibition of EphA2 and c-MET or both and HGF as a chemotactic factor on MDA-MB-231 cells.

(D) A schematic representation of the mammosphere formation assay.

(E) Mammosphere formation assay post knockdown/inhibition of c-MET or EphA2 or both in the presence of HGF.

(F) Matrigel tube formation assay post knockdown/inhibition of c-MET or EphA2 or both in the presence of HGF.

(G) Representative photomicrograph of freshly harvested breast tumors with a ruler (below) for scale.

(H) Graph representing the tumor volume vs. the various groups. Data are representative of triplicate experiments (mean ± SD). ∗p < 0.05 statistically significant difference compared to corresponding control by one-way ANOVA. Scale bars: 100 μm. Also see, Figures S3 and S4.