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. 2023 Nov 4;26(12):108395. doi: 10.1016/j.isci.2023.108395

Figure 4.

Figure 4

Induction of apoptosis by the combination regimen of Lupeol and 5FU on MDA-MB-231 cells

(A) Annexin V/PI apoptosis assay by flow cytometry post treatment with Lupeol or 5FU or both on MDA-MB-231 cells (in the presence of HGF).

(B) Graph representing the total apoptotic cells (%) for various treatment arms of MDA-MB-231.

(C) Immunofluorescence staining to detect Bax protein (exhibiting red fluorescence) post treatment with Lupeol and 5FU or both on MDA-MB-231 cells (in the presence of HGF).

(D) Graph representing the corrected total cell fluorescence (CTCF) of various treatment arms of MDA-MB-231 cells.

(E) Western blot analysis probed for Bax and Bcl2 proteins post treatment with Lupeol or 5FU or both on MDA-MB-231 cells (in the presence of HGF).

(F) Graph representing the normalized band intensity of Bax protein of various treatment arms of MDA-MB-231 cells.

(G) Graph representing the normalized band intensity of Bcl2 protein of various treatment arms of MDA-MB-231 cells. Data are representative of triplicate experiments (mean ± SD). ∗p < 0.05 and ∗∗∗p < 0.001 statistically significant difference compared to corresponding control by one-way ANOVA. HGF = hepatocyte growth factor; HF = HGF+5FU; HL = HGF+Lupeol; HFL = HGF+5FU + Lupeol. Scale bars: 100 μm.