Figure 7.

Evaluation of the combinatorial effect of 5FU and Lupeol in ex vivo explant culture model

(A) Graphical representation of the patient data used in this experiment, pertaining to the nodal status and tumor stage.

(B) Representative images of IHC staining of TNBC tissue fragments, post exposure to HGF, 5FU, Lupeol alone or in combination cells for the detection of Ki67 and Caspase 3c protein expression (Scale bars: 200 μm).

(C) Graph representing the percentage of cells expressing Ki67 vs. the various treatment arms.

(D) Graph representing the IHC score of Caspase 3c vs. various treatment arms.

(E) Representative images of IHC staining for the evaluation of phospho-EphA2 and phospho-cMET in various treatment arms (Scale bars: 200 μm and 100 μm in inset).

(F) Graph representing the various IHC scores of phospho-EphA2 in various treatment arms.

(G) Graph representing the various IHC scores of phospho-cMET in various treatment arms.

(H) Graph representing the various IHC scores of Caspase 3c expression in TNBC tumors (N = 7) after treating them with various SOC and 5FU and Lupeol combination. Data are representative of triplicate experiments (mean ± SD). ∗p < 0.05 statistically significant difference compared to corresponding control by one-way ANOVA. HGF = hepatocyte growth factor; HF = HGF+5FU; HL = HGF+Lupeol; HFL = HGF+5FU + Lupeol. Also see, Table S9.