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. 2023 Nov 4;26(12):108395. doi: 10.1016/j.isci.2023.108395
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit polyclonal Anti-human/mouse Ki67 Affinity Biosciences Cat# AF0198; RRID: AB_2834152
Rabbit Anti-human/mouse Cleaved-Caspase 3 (Asp175), p17 Affinity Biosciences Cat# AF7022; RRID: AB_2835326
Rabbit polyclonal Anti human/mouse MET (C-28) Santa Cruz Biotechnology Cat# sc-161; RRID: AB_631941
Rabbit polyclonal Anti human/mouse p-MET (Tyr 1349) Santa Cruz Biotechnology Cat# sc-34086; RRID: AB_2144033
Rabbit Anti human/mouse EphA2 (D4A2) Cell Signaling Technology Cat#6997; RRID: AB_10827743
Rabbit Anti human/mouse Phospho-EphA2 (Ser897) (D9A1) Cell Signaling Technology Cat#6347; RRID: AB_11220420
Rabbit Anti human/mouse Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Cell Signaling Technology Cat#4370; RRID: AB_2315112
Mouse Anti human/mouse MMP-2 (clone 8B4) Novus Cat# NB200-114; RRID: AB_350369
mouse Anti human Laminin- 5 (Ƴ2 chain) (D4B5) Millipore Cat#MAB19562; RRID: AB_94454
Rabbit polyclonal anti -Bcl2, unconjugated Novus Biologicals Cat# NB100-56101; RRID: AB_2064432
mouse monoclonal anti-Bax (Clone: B9) Santa Cruz Biotechnology Cat # sc-7480; RRID: AB_626729
Rabbit polyclonal Anti E-cadherin (H-108) Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666
Mouse monoclonal Anti human Vimentin (V9) Santa Cruz Biotechnology Cat# sc-6260; RRID: AB_628437
Rabbit polyclonal Anti- human ERK1+ERK2 antibody Abcam Cat# ab17942; RRID: AB_2297336
Mouse anti human/mouse Twist-1 (10E4E6) Novus Cat# NBP2-37364; RRID: AB_2801339
Mouse monoclonal anti human slug (4B6D5) Thermo Fisher Scientific Cat# MA5-38634; RRID: AB_2898546
Mouse Anti human Snail1 (20C8) Thermo Fisher Scientific Cat# 14-9859-82; RRID: AB_2573045
Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 31460; RRID: AB_228341
Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP. Thermo Fisher Scientific Cat# # 61-6520; RRID: AB_2533933

Biological samples

Human CaBr tumor material Chittaranjan National Cancer Institute, Hospital wing Collected per A-4.311/NM/26/11/2018

Chemicals, peptides, and recombinant proteins

Fetal Bovine serum Gibco Cat# 10438026
0.25% Trypsin-EDTA (1X) Gibco Cat# 25200072
Dulbecco’s Modified Eagle Medium powder Gibco Cat# 12800017
RPMI Medium 1640 Gibco Cat# 31800022
Anti-Anti (100X) Gibco Cat# 15240062
Matrigel Matrix Basement Membrane, Growth Factor Reduced, Phenol red free Corning Cat# 356231
MTT Merck Cat# 475989
Dimethyl Sulfoxide Sigma Cat# D2650
Gram’s crystal violet solution Merck Cat# 10921801251730
Insulin solution from bovine pancreas Sigma Cat# I0516
Formaldehyde solution Merck Cat# 1.94900.0521
Lupeol Sigma-Aldrich Cat# S957712
5FU Sigma-Aldrich Cat# F6627
4-hydroperoxy cyclophosphamide Cayman Cat#19527
Epirubicin Hydrochloride BioVision Cat# 1753
Docetaxel BioVision Cat# 2232
Doxorubicin.HCL BioVision Cat#1527
Paclitaxel Sigma-Aldrich Cat#T1912
Human HGF Sigma-Aldrich Cat# H9661
Recombinant mouse HGF protein R&D Systems Cat# 2207-HG-025/CF
B-27™ Supplement (50X), serum free Gibco Cat# 17504044
ALW-II-41-27 Cayman Cat# 1186206-79-0
Cabozantinib APExBIO Cat# A2977
XenoLight D-Luciferin, Potassium salt Perkin-Elmer Cat# 122799

Critical commercial assays

jetPRIME Transfection Reagent Polyplus Cat# 11407
In vivo-jetPEI Polyplus Cat# 201
IHC Select, Immunoperoxidase Secondary Detection System Millipore Cat# DAB150
Annexin V Apoptosis Detection Kit Santa Cruz Cat# sc-4252 AK

Experimental models: Cell lines

MDA MB 231 NCCS, Pune, India
4T1 Kindly gifted by Dr. Rathindranath Baral (CNCI, India)

Experimental models: Organisms/strains

BALB/c (Female) State Centre for Laboratory Animal Breeding, West Bengal Livestock Development Corporation Limited

Oligonucleotides

ON-TARGET plus Human MET (4233) siRNA- SMARTpool Dharmacon Cat# L-003156-00-0005
ON-TARGETplus Human EphA2 (1969) siRNA-SMARTpool Dharmacon Cat# L-003116-00-0005
ON-TARGET plus Mouse Met (17295) siRNA- SMARTpool Dharmacon Cat# L-040878-00-0005
ON-TARGET plus Mouse EphA2 (13836) siRNA- SMARTpool Dharmacon Cat# L-040412-00-0005
ON-TARGETplus Non-targeting Pool Dharmacon Cat# D-001810-10-05

Software and algorithms

FlowJo 10.8.1 BD Biosciences
GraphPad Prism 7.0 GraphPad software Inc.
SPSS SPSS Inc.
Fiji ImageJ
AngioTool v0.6a Zudaire et al.53
SynergyFinder 3.0 Ianevski et al.54