Antibodies |
|
Rabbit polyclonal Anti-human/mouse Ki67 |
Affinity Biosciences |
Cat# AF0198; RRID: AB_2834152
|
Rabbit Anti-human/mouse Cleaved-Caspase 3 (Asp175), p17 |
Affinity Biosciences |
Cat# AF7022; RRID: AB_2835326
|
Rabbit polyclonal Anti human/mouse MET (C-28) |
Santa Cruz Biotechnology |
Cat# sc-161; RRID: AB_631941
|
Rabbit polyclonal Anti human/mouse p-MET (Tyr 1349) |
Santa Cruz Biotechnology |
Cat# sc-34086; RRID: AB_2144033
|
Rabbit Anti human/mouse EphA2 (D4A2) |
Cell Signaling Technology |
Cat#6997; RRID: AB_10827743
|
Rabbit Anti human/mouse Phospho-EphA2 (Ser897) (D9A1) |
Cell Signaling Technology |
Cat#6347; RRID: AB_11220420
|
Rabbit Anti human/mouse Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP |
Cell Signaling Technology |
Cat#4370; RRID: AB_2315112
|
Mouse Anti human/mouse MMP-2 (clone 8B4) |
Novus |
Cat# NB200-114; RRID: AB_350369
|
mouse Anti human Laminin- 5 (Ƴ2 chain) (D4B5) |
Millipore |
Cat#MAB19562; RRID: AB_94454
|
Rabbit polyclonal anti -Bcl2, unconjugated |
Novus Biologicals |
Cat# NB100-56101; RRID: AB_2064432
|
mouse monoclonal anti-Bax (Clone: B9) |
Santa Cruz Biotechnology |
Cat # sc-7480; RRID: AB_626729
|
Rabbit polyclonal Anti E-cadherin (H-108) |
Santa Cruz Biotechnology |
Cat# sc-7870; RRID: AB_2076666
|
Mouse monoclonal Anti human Vimentin (V9) |
Santa Cruz Biotechnology |
Cat# sc-6260; RRID: AB_628437
|
Rabbit polyclonal Anti- human ERK1+ERK2 antibody |
Abcam |
Cat# ab17942; RRID: AB_2297336
|
Mouse anti human/mouse Twist-1 (10E4E6) |
Novus |
Cat# NBP2-37364; RRID: AB_2801339
|
Mouse monoclonal anti human slug (4B6D5) |
Thermo Fisher Scientific |
Cat# MA5-38634; RRID: AB_2898546
|
Mouse Anti human Snail1 (20C8) |
Thermo Fisher Scientific |
Cat# 14-9859-82; RRID: AB_2573045
|
Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP |
Thermo Fisher Scientific |
Cat# 31460; RRID: AB_228341
|
Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP. |
Thermo Fisher Scientific |
Cat# # 61-6520; RRID: AB_2533933
|
|
Biological samples |
|
Human CaBr tumor material |
Chittaranjan National Cancer Institute, Hospital wing |
Collected per A-4.311/NM/26/11/2018 |
|
Chemicals, peptides, and recombinant proteins |
|
Fetal Bovine serum |
Gibco |
Cat# 10438026 |
0.25% Trypsin-EDTA (1X) |
Gibco |
Cat# 25200072 |
Dulbecco’s Modified Eagle Medium powder |
Gibco |
Cat# 12800017 |
RPMI Medium 1640 |
Gibco |
Cat# 31800022 |
Anti-Anti (100X) |
Gibco |
Cat# 15240062 |
Matrigel Matrix Basement Membrane, Growth Factor Reduced, Phenol red free |
Corning |
Cat# 356231 |
MTT |
Merck |
Cat# 475989 |
Dimethyl Sulfoxide |
Sigma |
Cat# D2650 |
Gram’s crystal violet solution |
Merck |
Cat# 10921801251730 |
Insulin solution from bovine pancreas |
Sigma |
Cat# I0516 |
Formaldehyde solution |
Merck |
Cat# 1.94900.0521 |
Lupeol |
Sigma-Aldrich |
Cat# S957712 |
5FU |
Sigma-Aldrich |
Cat# F6627 |
4-hydroperoxy cyclophosphamide |
Cayman |
Cat#19527 |
Epirubicin Hydrochloride |
BioVision |
Cat# 1753 |
Docetaxel |
BioVision |
Cat# 2232 |
Doxorubicin.HCL |
BioVision |
Cat#1527 |
Paclitaxel |
Sigma-Aldrich |
Cat#T1912 |
Human HGF |
Sigma-Aldrich |
Cat# H9661 |
Recombinant mouse HGF protein |
R&D Systems |
Cat# 2207-HG-025/CF |
B-27™ Supplement (50X), serum free |
Gibco |
Cat# 17504044 |
ALW-II-41-27 |
Cayman |
Cat# 1186206-79-0 |
Cabozantinib |
APExBIO |
Cat# A2977 |
XenoLight D-Luciferin, Potassium salt |
Perkin-Elmer |
Cat# 122799 |
|
Critical commercial assays |
|
jetPRIME Transfection Reagent |
Polyplus |
Cat# 11407 |
In vivo-jetPEI |
Polyplus |
Cat# 201 |
IHC Select, Immunoperoxidase Secondary Detection System |
Millipore |
Cat# DAB150 |
Annexin V Apoptosis Detection Kit |
Santa Cruz |
Cat# sc-4252 AK |
|
Experimental models: Cell lines |
|
MDA MB 231 |
NCCS, Pune, India |
|
4T1 |
|
Kindly gifted by Dr. Rathindranath Baral (CNCI, India) |
|
Experimental models: Organisms/strains |
|
BALB/c (Female) |
State Centre for Laboratory Animal Breeding, West Bengal Livestock Development Corporation Limited |
|
|
Oligonucleotides |
|
ON-TARGET plus Human MET (4233) siRNA- SMARTpool |
Dharmacon |
Cat# L-003156-00-0005 |
ON-TARGETplus Human EphA2 (1969) siRNA-SMARTpool |
Dharmacon |
Cat# L-003116-00-0005 |
ON-TARGET plus Mouse Met (17295) siRNA- SMARTpool |
Dharmacon |
Cat# L-040878-00-0005 |
ON-TARGET plus Mouse EphA2 (13836) siRNA- SMARTpool |
Dharmacon |
Cat# L-040412-00-0005 |
ON-TARGETplus Non-targeting Pool |
Dharmacon |
Cat# D-001810-10-05 |
|
Software and algorithms |
|
FlowJo 10.8.1 |
BD Biosciences |
|
GraphPad Prism 7.0 |
GraphPad software Inc. |
|
SPSS |
SPSS Inc. |
|
Fiji |
ImageJ |
|
AngioTool v0.6a |
|
Zudaire et al.53
|
SynergyFinder 3.0 |
|
Ianevski et al.54
|