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. 2023 Oct 20;299(12):105369. doi: 10.1016/j.jbc.2023.105369

Figure 1.

Figure 1

cMyBP-C organization in the sarcomere.A, top, the sarcomere spans from Z-disc to Z-disc, with the A-band containing thick filaments and the I-band containing actin filaments. Force is generated by myosin and actin in the thin/thick filament overlap portion of the A-band. cMyBP-C molecules (green vertical stripes) are anchored to the thick filament and present in the C-zones toward the center of the A-band. The C-zones overlap with thin filaments (except at very long sarcomere lengths). Adapted from (21). Bottom: Full-length cMyBP-C domains C0 through C10. Ig-like domains are shown as circles and fibronectin type-III domains are shown as hexagons. N-terminal domains C0 through C2 (C0-C2), contain the flexible proline alanine-rich linker (P/A) and the partially disordered M-domain (M) that contains phosphorylation sites (P). Skeletal MyBP-C does not have C0. B, FMAL-actin and TMR-cC0-C2/fC1-C2 biosensor. The TMR label is located in the C1 domain. C and D, fluorescence waveform of FMAL-actin shows a faster decay in the presence of acceptor-labeled protein (red curve) than in the absence (blue curve), indicating FRET. Adapted from (13).