Figure 4.

A representative screen of the Selleck library. Screening was done in duplicate with two different preparations of FMAL-actin and TMR-C0-C2 and reproducible hits were identified. Hits are identified as exceeding a z-score of ±4 of the plate-wide statistics. A, excitation at 473 nm. Reproducible donor-only (dark gray dots) and donor-acceptor (DA) (blue) hits at 473 nm. Donor-only hits are excluded from the ‘hits’ list as they affect the lifetime of FMAL-actin directly. Fluorescent compounds are shown in black and compounds that do not change FLT beyond the hit threshold are shown in light grey. B, excitation at 532 nm. Reproducible hits changing the FLT of TMR on cC0-C2 are shown as orange dots.