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. 2023 Oct 19;299(12):105367. doi: 10.1016/j.jbc.2023.105367

Figure 2.

Figure 2

C-CAP1 promotes depolymerization of ADP-Piactin filaments.A, rates of barbed end depolymerization at different concentrations of C-CAP1 in mf-TIRF assays. The data (mean ± SD) are from three independent replicates (n = 30 filaments total per condition). The line is a fit to a hyperbolic binding curve and was used to derive the Kapp (0.85 μM). B, rates of barbed end depolymerization at different concentrations of C-CAP1 in mf-TIRF assays in the presence of 50 mM inorganic phosphate (Pi). Data (mean ± SD) are from two independent replicates (n = 20 filaments total per condition). The line is a fit to a hyperbolic binding curve and was used to derive the Kapp (1.74 μM). CAP, cyclase-associated protein; C-CAP, C-terminal half of CAP; mf-TIRF, microfluidics-assisted total internal reflection fluorescence.