Table 2.
Effect of various concentrations of TDZ, kinetin, and BAP on embryo maturation of calli from immature leaves of Momordica charantia L.
| Sr. No |
Growth regulators | Concentration (mg.L−1) | Percentage of embryogenic calli ± S.E |
Nature of embryogenic calli |
|||
|---|---|---|---|---|---|---|---|
| Callus-derived from immature leaf of in vitro plantlets | Callus-derived from immature leaf of in vivo plants | Callus-derived from immature leaf of in vitro plantlets | Callus-derived from immature leaf of in vivo plant | ||||
| 1 | TDZ + KIN | 0.8 + 0.0 | 75.25 ± 0.25d | 67.26 ± 0.20d | LYGOC | GYGC | |
| 2 | 0.8 + 0.5 | 80.25 ± 0.14c | 72.17 ± 0.08c | GYGC | MYGC | ||
| 3 | 0.8 + 0.8 | 66.19 ± 0.47e | 58.28 ± 0.15f | GYGC | GYGC | ||
| 4 | TDZ + BAP | 1.2 + 0.0 | 75.30 ± 0.19d | 66.27 ± 0.14e | BYGC | BYGC | |
| 5 | 1.2 + 0.5 | 100.00 ± 0.00a | 90.19 ± 0.10a | GYGF | BYGF | ||
| 6 | 1.2 + 0.8 | 91.19 ± 0.29b | 85.43 ± 0.22b | BYGC | BYGC | ||
| Mean | – | 81.36 ± 4.99 | 73.27 ± 5.72 | – | – | ||
| F Value | – | 2103.29 | 5868.16 | – | – | ||
| P-value | – | <0.0001 | <0.0001 | – | – | ||
| LSD (0.05) | – | 0.82 | 0.49 | – | – | ||
| CV,% | – | 0.57 | 0.38 | – | – | ||
| R2 | – | 0.02 | 0.01 | – | – | ||
Values are the mean ± Standard deviation (S.E) of 12 replications per treatment, and all experiments were conducted thrice. No difference between means with the same letter when compared with Tukey multiple comparison method. LYGOC light yellow-green olive compact, GYGC greyish yellow green compact, BYGC brilliant yellow-green compact, GYGF greyish yellow green friable, MYGC moderate yellow green compact, brilliant yellow-green friable (Royal Horticultural Society colour chart, London, UK, sixth edition, 2015).