Table 3.
Effect of various concentrations of BAP with TDZ, 2,4-D, and NAA on shoot bud formation from immature leaves of (Momordica charantia).
| Sr. No |
Growth regulators | Concentration (mg.L−1) | Percent of calli formed shoots ± S.E |
Shoots length (cm) ± S.E |
|||
|---|---|---|---|---|---|---|---|
| Immature leaf of in vitro plantlets | Immature leaf of in vivo plants | Immature leaf of in vitro plantlets | Immature leaf of in vivo plants | ||||
| 1 | BAP | 0.00 | 90.91 ± 2.62a | 68.18 ± 1.95a | 7.13 ± 0.54a | 5.63 ± 0.54 a | |
| 2 | 0.50 | 46.97 ± 1.15e | 24.24 ± 1.51f | 2.56 ± 0.65d | 1.43 ± 0.68d | ||
| 3 | 0.75 | 27.27 ± 2.54g | 7.57 ± 1.51g | 2.40 ± 0.45d | 1.23 ± 0.25de | ||
| 4 | 1.00 | 00.00 ± 00.00 h | 00.00 ± 00.00 h | 00.00 ± 00.00e | 00.00 ± 00.00e | ||
| 5 | 1.25 | 00.00 ± 00.00 h | 00.00 ± 00.00 h | 00.00 ± 00.00e | 00.00 ± 00.00e | ||
| 6 | BAP + 2,4-D | 0.50 + 0.25 | 70.59 ± 1.96c | 55.88 ± 1.57c | 3.61 ± 0.24cd | 1.86 ± 0.97cd | |
| 7 | 0.75 + 0.50 | 35.29 ± 1.69f | 20.58 ± 1.69f | 2.85 ± 0.32d | 1.60 ± 1.25d | ||
| 8 | 1.00 + 0.75 | 00.00 ± 00.00 h | 00.00 ± 00.00 h | 00.00 ± 00.00e | 00.00 ± 00.00e | ||
| 9 | BAP + TDZ | 0.50 + 0.25 | 76.47 ± 1.58b | 61.76 ± 1.49b | 5.36 ± 0.58b | 3.83 ± 0.38b | |
| 10 | 0.75 + 0.50 | 44.44 ± 1.60e | 30.55 ± 1.95e | 3.40 ± 0.54cd | 3.83 ± 0.62cd | ||
| 11 | 1.00 + 0.75 | 00.00 ± 00.00 h | 00.00 ± 00.00 h | 00.00 ± 00.00e | 00.00 ± 00.00e | ||
| 12 | BAP + NAA | 0.50 + 0.25 | 73.68 ± 1.15BCE | 60.52 ± 1.89b | 4.52 ± 0.29BCE | 3.03 ± 0.57BCE | |
| 13 | 0.75 + 0.50 | 57.90 ± 1.51d | 44.73 ± 1.38d | 3.34 ± 0.77cd | 2.03 ± 0.43cd | ||
| 14 | 1.00 + 0.75 | 00.00 ± 00.00 h | 00.00 ± 00.00 h | 00.00 ± 00.00e | 00.00 ± 00.00e | ||
| Mean | – | 37.39 ± 4.50 | 26.71 ± 6.65 | 2.51 ± 0.61 | 1.62 ± 0.33 | ||
| F Value | – | 488.35 | 365.04 | 26.42 | 139.43 | ||
| P-value | – | <0.0001 | <0.0001 | <0.0001 | <0.0001 | ||
| LSD (0.05) | – | 4.37 | 4.04 | 1.28 | 1.30 | ||
| CV,% | – | 6.99 | 9.05 | 30.61 | 17.40 | ||
| R2 | 0.59 | 0.48 | 0.61 | 0.60 | |||
Values are the mean ± Standard deviation (S.E) of 12 replications per treatment and all experiments conducted thrice. No difference between means with the same letter when compared with Tukey multiple comparison method.