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Fluorescence quenching of F–M-labeled cells by ferrichrome. E. coli UL3 fhuA transformed with plasmid pfhuA8 [FhuA(wild type)], pfhuA5 [FhuA (C329S) Cys-318], pfhuA4 [FhuA(C318S) Cys-329], pfhuA6 [FhuA (D336C)], or pfhuA7 [FhuA(V347C)] was labeled with F-M. The fluorescence was determined by flow cytometry prior to (−) and after (…) the addition of 10 nM ferrichrome (A) or 1 μM ferrioxamine B {pfhuA6 [FhuA(D336C)]} (B).
