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E. coli K-12 strains and plasmids used in this study
| Strains and plasmids | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| UL3 | AB2847 fhuA recA Tn10 | 16 |
| BR158 | AB2847 tonB | 13 |
| HK99 | BR158 fhuA | 16 |
| Plasmids | ||
| pT7-6 | Ampr | 39 |
| pHK763 | pT7-6 FhuA wild type | 16 |
| pfhuA4 | pT7-6 FhuA(C318S) | 6 |
| pfhuA5 | pT7-6 FhuA(C329S) | 6 |
| pfhuA6 | pT7-6 FhuA(D336C) | This study |
| pfhuA7 | pT7-6 FhuA(V347C) | This study |
| pfhuA8 | pHK763 with an additional BamHI site | This study |
| pfhuA9 | pT7-6 FhuA(C318S C329S C692S C698S) | 6 |
