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Specific fluorescence quenching by ferrichrome
| FhuA | Relative fluorescence (mean values)a
|
||||
|---|---|---|---|---|---|
| Not treated with Fc | Treated with Fc | −ΔF (%) | FoB | −ΔF (%) | |
| Wild type | 48 | 45 | 6 | 50 | +2 |
| C329S (Cys-318) | 76 | 64 | 16 | 71 | 7 |
| C318S (Cys-329) | 97 | 70 | 27 | 100 | +3 |
| D336C | 405 | 262 | 35 | 385 | 5 |
| V347C | 154 | 141 | 8 | 145 | 6 |
−ΔF (%) indicates the decrease or increase (+) in the percentage of fluorescence for cells not treated or treated with 10 nM ferrichrome (Fc) or treated with 1 μM ferrioxamine B (FoB).
