TABLE 3.
Inhibition of cell labeling by ferrichrome
| FhuA | Relative fluorescence (mean values)a
|
||
|---|---|---|---|
| Not preincubated with Fc | Preincubated with Fc | −δF (%) | |
| C329S (Cys-318) | 45 | 38 | 16 |
| C318S (Cys-329) | 61 | 51 | 16 |
| D336C | 196 | 186 | 5 |
| V347C | 90 | 52 | 42 |
−δF (%) indicates the decrease in F-M labeling for cells not preincubated with 100 μM ferrichrome (Fc) (standard deviation, 10%).