Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sequence of Primers for qPCR assay in mice.
| Gene | Sequence (5′—3′) |
|---|---|
| IL-1β | F：TGCCACCTTTTGACAGTGATG |
| R：TGATGTGCTGCTGCGAGATT | |
| IL-6 | F：CCCCAATTTCCAATGCTCTCC |
| R：CGCACTAGGTTTGCCGAGTA | |
| TNF-α | F：GATCGGTCCCCAAAGGGATG |
| R：TTTGCTACGACGTGGGCTAC | |
| NLRP3 | F：ATTACCCGCCCGAGAAAGG |
| R： TCGCAGCAAAGATCCACACAG | |
| PKM2 | F：GCCGCCTGGACATTGACTC |
| R：CCATGAGAGAAATTCAGCCGAG | |
| GAPDH | F：AGGTCGGTGTGAACGGATTTG |
| R：TGTAGACCATGTAGTTGAGGTCA |
