FIG. 2.

Genetic organization of the Mx8 immunity region. The 19 potential genes in the sequenced immunity region are transcribed from the conventional top strand, from left to right. The four subsets of genes, imm-2-3-4, 8-9-10, 13-14, and 15-16-17-18, are arranged so that 2 bp of the stop and start codons of adjacent genes within each cluster are shared. Below the gene map are shown the endpoints of plasmid inserts used to define the determinants of superinfection immunity and site-specific recombination. Restriction sites: Sau3AI (S; bp 1), EcoRI (R; bp 1897 and 2761), XhoI (X; bp 628, 658, 3687, and 9476), MfeI (M; bp 4585), and PvuII (V; bp 8069). The pairs of EcoO109 sites used to generate the deletions in pAY258 and pAY259 are at bp 504 and 2922 and at bp 504 and 3805, respectively. The deletion between BlpI sites in pAY456 extends from bp 278 to 4238. Bars are as described in the legend for Fig. 1.