TABLE 2.
Potential ORFs in the Mx8 imm/int regiona
| Potential gene | Designation | Frame | Interval | %G+C | RBS | Apparent Mr |
|---|---|---|---|---|---|---|
| 1 | imm(110) | 2α | 35..367 | 83.5 | TAAGGAGGG (5) | 12,426 |
| 2 | ORF 265 | 1α | 364..1158 | 88.3 | GAAGGGCGG (6) | 28,439 |
| 3 | ORF 105 | 3α | 1155..1472 | 86.6 | CGAGAGCGT (5) | 11,377 |
| 4 | ORF 59 | 2α | 1469..1648 | 84.5 | GACGAAGGG (6) | 6,532 |
| 5 | mox(258) | 1 | 1651..2427 | 86.8 | AGAGGAGGC (6) | 28,733 |
| 6 | ORF 75 | 1 | 2533..2760 | 75.7 | AGTGGGGGT (4) | 8,175 |
| 7 | orf 107 | 2 | 2870..3193 | 84.9 | CCGGGAGGA (4) | 13,751 |
| 8 | ORF 150 | 3β | 3294..3746 | 80.5 | CATGGAGCC (4) | 17,203 |
| 9 | ORF 100 | 2β | 3743..4045 | 82.8 | ACGCGAGGC (5) | 10,596 |
| 10 | ORF 80 | 1β | 4042..4284 | 82.3 | AGAGTTGGT (5) | 8,726 |
| 11 | uoi (74) | 2 | 4991..5215 | 75.3 | GAAGACGGA (4) | 7,845 |
| 12 | int(574) | 3 | 5085..6809 | 80.5 | GACGGCTGG (5) | 64,471 |
| 13 | ORF 68 | 2γ | 6857..7063 | 82.1 | AGAGGAGTC (4) | 7,219 |
| 14 | ORF222 | 1γ | 7060..7728 | 91.9 | GCGCGGGGT (5) | 25,257 |
| 15 | ORF130 | 2δ | 7898..8287 | 73.4 | CAAGGGGCA (7) | 13,913 |
| 16 | ORF93 | 1δ | 8284..8562 | 89.0 | TCCGGAGGG (4) | 10,504 |
| 17 | ORF104 | 3δ | 8559..8873 | 89.3 | GAAGGCGGT (6) | 11,785 |
| 18 | ORF106 | 2δ | 8870..9190 | 94.3 | GCAGGAGGG (6) | 11,834 |
| 19 | ORF(>68) | 2 | 9278..>9481 | 85.1 | TGAGGGAGT (7) | >7,869 |
Gene 15 is included because it begins an overlapping array of genes, even though it has <75% G or C in the third position. Four of these genes, imm, mox, uoi, and int, with functions suggested from our genetic analysis, are given three-letter names, followed by the number of amino acid residues in their predicted products. Other potential ORFs are assigned numbers corresponding to the numbers of amino acid residues in their products, including the N-terminal methionine residues. We assume that ORFs which start with GTG will have N-terminal methionines. (Although many mycophage L5 ORFs begin with TTG start codons [18], we did not find these potential starts for Mx8 genes.) The coordinates of the top strand, translation frames, and gene intervals are as in GenBank accession no. U64984. Four sets of genes are organized as overlapping arrays, designated α, β, γ, and δ. The percent G+C occurrence is for the third codon position, not including codons for N-terminal methionine residues. Ribosome-binding sites (RBS) were assigned as the best-fit similarities (underlined) to the sequence TAAGGAGGT, complementary to the 3′ end of M. xanthus 16S rRNA (41). Numbers in parentheses after each RBS indicate the spacing between the homology shown and the start codon for each ORF.