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. 2023 Oct 21;299(12):105379. doi: 10.1016/j.jbc.2023.105379

Figure 3.

Figure 3

AMPKα1 deletion inhibits mitochondrial fission.A, representative images of Ctskcre/0 and Ctskcre/0AMPKα1f/f osteoclast differentiation by TRAP staining. The scale bar corresponds to 200 μm. B, quantification of osteoclast number and area in vitro. C, protein expression of osteoclasts and mitochondrial dynamics markers: NFATc1, DC-STAMP, mitofusin 2, Drp-1, and β-actin as positive control. D, representative image of Ctskcre/0 and Ctskcre/0AMPKα1f/f osteoclasts labeled with MitoTracker Red. The scale bar corresponds to 200 μm. (n = 5, Student's t test ∗p < 0.05 and ∗∗p < 0.01). All experiments were performed two times. AMPKα1, AMP-activated protein kinase α1; Drp1, dynamics-related protein 1; TRAP, tartrate-resistant acid phosphatase.