Fig. 3.

MIOX regulates autophagy and EMT in ccRCC cells. (A-D) Representative images of LC3 fluorescence of 786-O (A) and 769-P (C) cells in different treatment groups, and statistical results of LC3 puncta in 786-O (B) and 769-P (D) cells. Starvation was for 2 h. Scale bar: 5 μm. (E) TEM images to indicate the changes of autophagosome and autolysosome after MIOX overexpression in ccRCC cell lines. Yellow arrow: autophagosome, red arrow: autolysosome. Scale bar: 1 μm. (F) The top 10 gene sets significantly enriched in MIOX low expression group in the TCGA ccRCC cohort. (G) GSEA results of EMT pathway-related genes changing with MIOX expression in TCGA ccRCC cohort (normalized enrichment score (NES) = -1.91, p < 0.01). (H) Representative images of morphology of cells with the MIOX overexpression or knockdown. (I) Representative images of immunofluorescence staining for E-cadherin after MIOX overexpression or knockdown in 786-O and 769-P cells. Scale bar: 20 μm. (J-M) The protein expression and quantitative result (n = 3) of autophagy- and EMT-related markers after overexpression (J and L) or knockdown (K and M) of MIOX in ccRCC cell lines. *, p < 0.05, **, p < 0.01, ***, p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)