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. 2023 Nov 7;68:102956. doi: 10.1016/j.redox.2023.102956

Fig. 5.

Fig. 5

Elevated ROS levels resulted from inhibition of autophagy by MIOX leads to the inhibition of the STAT3/c-Myc-mediated EMT and aggressiveness in ccRCC cells. (A) The top 10 gene sets significantly enriched in MIOX-control cells from the RNA-seq data. (B–C) GSEA results of MYC (B) and IL6-JAK-STAT3 (C) pathway-related genes in MIOX-control and MIOX-overexpression cells (Con vs MIOX) form the RNA-seq data (MYC: NES = −2.06, p < 0.001; IL6-JAK-STAT3: NES = −1.64, p < 0.001). (D-E) The protein expression of p-STAT3, STAT3 and c-Myc in 786-O (D) and 769-P (E) cells with stable overexpression of MIOX. (F-G) The levels of ROS in MIOX-overexpression 786-O (F) and 769-P (G) cells detected by flow cytometry. Rapamycin: 400 nM for 24 h (n = 3). (H–I) The effect of NAC on the expression of p-STAT3, STAT3 and c-Myc in MIOX-overexpression 786-O (H) and 769-P (I) cells. NAC: 10 mM for 24 h. (J-K) The effect of Colivelin and 10058-F4 on the expression of E-cadherin and Vimentin (J) and migration and invasion (K) in MIOX-overexpression 786-O cells. Colivelin: 1 μM for 24 h. 10058-F4: 20 μM for 24 h. (L-M) The effect of Colivelin and 10058-F4 on the expression of E-cadherin and Vimentin (L) and migration and invasion (M) in MIOX-overexpression 769-P cells. Colivelin: 1 μM for 24 h. 10058-F4: 20 μM for 24 h *, p < 0.05, **, p < 0.01, ***, p < 0.001.