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. 2023 Nov 7;68:102956. doi: 10.1016/j.redox.2023.102956

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Overexpression of MIOX inhibits RCC cell growth and metastasis in vivo. (A) The expression of FLAG label in Renca transfected with lentivirus carrying FLAG-labelled plasmid of MIOX overexpression by western blot. (B) Representative images of subcutaneous tumors in the MIOX control group and the overexpression group; n = 6/group. (C) The volume and weight of the subcutaneous tumors. (D) Representative living images of mice injected with Renca transfected by indicated lentivirus into tail vein. The lentivirus was Luci-labelled and therefore stably transfected Renca cell lines had in vivo luciferase activity. (E) Statistical analysis of luciferase bioluminescence intensity (n = 6). (F) Representative images of metastases in murine lung of each group; n = 5/group. The black arrow indicated the metastasis. (G) H&E staining of pulmonary tissue sections. Scale bar: 500 μm for images above and 100 μm for images below. (H) Statistical analysis of the number of pulmonary metastases of each group. (I) The protein expression of MIOX, LC3, SQSTM1, STAT3, p-STAT3 and c-Myc in pulmonary metastases of mice by IHC staining. Scale bar: 500 μm *, p < 0.05, **, p < 0.01, ***, p < 0.001.