Table 2.

ERFE-BMP dissociation rates (k_d (1/s) and log k, mean ± SD, n = 5)

ERFE variant	BMP2/6	BMP2	BMP6
Full length, 43-354	0.00022 ± 0.00014	0.00009 ± 0.00002	0.00022 ± 0.00018
	−3.73 ± 0.30	−4.05 ± 0.11	−3.79 ± 0.41
N terminus (mammalian), 43-148	0.0022 ± 0.0003	0.0050 ± 0.0003	0.0042 ± 0.0002
	−2.67 ± 0.05	−2.30 ± 0.02	−2.38 ± 0.02
N terminus (bacterial), 43-148	0.0051 ± 0.0002	0.0046 ± 0.0002	0.0052 + 0.0003
	−2.29 ± 0.02	−2.34 ± 0.02	−2.29 ± 0.03
W82A, 43-148	0.024 ± 0.001	0.016 ± 0.008	0.029 ± 0.008
	−1.63 ± 0.02	−1.84 ± 0.24	−1.56 ± 0.12
6KA mutant, 43-148	0.029 ± 0.001	0.026 ± 0.002	0.029 ± 0.002
	−1.54 ± 0.01	−1.59 ± 0.004	−1.53 ± 0.02
KR switch, 43-148	0.0068 ± 0.0002	0.0058 ± 0.0008	0.0069 + 0.0005
	−2.17 ± 0.01	−2.24 ± 0.06	−2.16 ± 0.03
ΔCollagen, 43-148 del109-125	0.0052 ± 0.0002	0.0056± 0.0031	0.0085 ± 0.0076
	−2.29 ± 0.02	−2.29 ± 0.20	−2.17 ± 0.30
ΔHelical, 43-125	0.033 ± 0.001	0.034± 0.002	0.045 + 0.002
	−1.48 ± 0.01	−1.47 ± 0.02	−1.35 ± 0.02
Segment 1, 43-95	no binding	no binding	no binding
Segment 2, 73-125	0.016 ±0.003	0.018 ± 0.004	0.027 + 0.005
	−1.79 ± 0.07	−1.76 ± 0.09	−1.57 ± 0.08
Segment 3, 96-148	0.019 ± 0.005	0.027 ± 0.004	0.032 + 0.005
	−1.74 ± 0.12	−1.57 ± 0.07	−1.50 ± 0.06