Fig. 2.

DHP-B suppresses colorectal cancer cell growth and induces apoptosis in vivo and improves survival of tumor-bearing mice. (A) Schematic illustration of the orthotopic colorectal cancer model and the treatment regimen; (B) Representative images and quantification of tumor weight from different treatment groups; (C) Immunofluorescence staining and quantification of Ki-67 (green) and cleaved caspase-3 (C-CASP3, red) in tumor tissues from different treatment groups (scale bar: 20 μm). The number of Ki-67 or C-CASP3 positive cells were counted using Image J 1.5.3 software. Data are median ± IQR from ten random fields per sample; (D) Kaplan-Meier survival curve of tumor-bearing mice treated with different agents. The blue box indicates the treatment period and the “+” sign denotes censored mice that were euthanized due to 20 % weight loss. The black dashed line represents the median survival time of each group. OXA was used as a positive control. Data are from eight mice per group. The above animal studies were evaluated using eight mice per group, with results reported as median ± IQR; NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t-test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)