Fig. 7.

CPT1A regulates CRC cell growth and survival in vivo. (A) Representative images and quantification of tumor weights of rectal tumors derived from SW620 cells with or without CPT1A knockdown (Vector and CPT1A^KD−1, respectively). (B) Fluorescence immunohistochemistry of Ki-67 (green) and C-CASP3 (red) expression, markers of cell proliferation and apoptosis, respectively, in tumor tissues with or without CPT1A knockdown (scale bar, 20 μm). Quantification of Ki-67 and C-CASP3 expression levels in different tumor tissues are shown in the boxplots. The expression ratios of Ki-67 and C-CASP3 were measured using Image J 1.5.3 software, and 10 random microscopic fields were counted and averaged for each sample; (C) Oil red O staining of lipid droplets in frozen tumor sections with or without CPT1A knockdown. Quantification of oil red O positive area ratios in different tumor tissues are shown in the boxplots. The positive area of oil red O was measured using Image J 1.5.3 software, and 10 random microscopic fields were counted and averaged for each sample; (D) Kaplan-Meier survival curve of NCG mice bearing orthotopic tumors derived from SW620 cells with or without CPT1A knockdown. The blue box represents the period of drug administration and the “+” sign denotes animals that did not perish but had a 20 % weight loss and were humanely euthanized. The black dashed line represents the median survival time of model animals. The animal studies were evaluated using seven mice per group, with results reported as median ± IQR; NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t-test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)