Characterization of iPSCs and neuroectodermal (NECT) colonies using immunocytochemistry
(A) Schematic diagram representing the protocol used to generate NECT colonies from stem cells. Representative bright-field-phase images showing the morphology of stem cells and NECT colonies in vitro are shown. Dotted white boxes indicate zoomed in regions. Scale bar = 500 μm, zoomed images scale bar = 100 μm.
(B) Immunocytochemistry staining of stem cell colonies marked with SOX2 (Green), NANOG (Red), OCT4 (Red), and N-CADHERIN (RED). Scale bar = 100 μm.
(C) Immunocytochemistry staining of NECT colonies marked with SOX2 (Green), NANOG (Red), OCT4 (Red), and N-CADHERIN (RED). Scale bar = 50 μm.