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. 2023 Nov 16;4(4):102725. doi: 10.1016/j.xpro.2023.102725

Figure 1.

Figure 1

Characterization of iPSCs and neuroectodermal (NECT) colonies using immunocytochemistry

(A) Schematic diagram representing the protocol used to generate NECT colonies from stem cells. Representative bright-field-phase images showing the morphology of stem cells and NECT colonies in vitro are shown. Dotted white boxes indicate zoomed in regions. Scale bar = 500 μm, zoomed images scale bar = 100 μm.

(B) Immunocytochemistry staining of stem cell colonies marked with SOX2 (Green), NANOG (Red), OCT4 (Red), and N-CADHERIN (RED). Scale bar = 100 μm.

(C) Immunocytochemistry staining of NECT colonies marked with SOX2 (Green), NANOG (Red), OCT4 (Red), and N-CADHERIN (RED). Scale bar = 50 μm.