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. 2023 Nov 16;4(4):102725. doi: 10.1016/j.xpro.2023.102725

Figure 5.

Figure 5

Characterization of neural spheroids at day 7 of in vitro differentiation

(A) Whole-mount immunostaining of neural spheroids stained with ZO1 (green) and SOX1 (red). All tissues were counterstained with Hoechst 33342 (blue). Scale bar = 100 μm.

(B) Whole-mount immunostaining of neural spheroids stained with SOX2 (green) and N-CADHERIN (red). All tissues were counterstained with Hoechst 33342 (blue). Scale bar = 100 μm.

(C) Whole-mount immunostaining of neural spheroids stained with SOX2 (green) and caudal spinal cord marker HOXC9 (red). All tissues were counterstained with Hoechst 33342 (blue). Scale bar = 100 μm.