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. 2023 Nov 16;4(4):102725. doi: 10.1016/j.xpro.2023.102725

Figure 6.

Figure 6

Characterization of differentiating neural spheroids in vitro

(A) Whole-mount immunostaining of differentiated neural spheroids at day 9 stained with N-CADHERIN (red) showing the neural rosettes and neural tube folding (white stars). All organoids were counterstained with Hoechst 33342 (blue). Scale bar = 50 μm.

(B) Whole-mount immunostaining of neural spheroids derived from WTC iPSCs-SOX10GFP reporter line, tissue was stained with SOX10 (red) and GFP (green). All organoids were counterstained with Hoechst 33342 (blue). Scale bar = 50 μm.

(C) Whole-mount 3D reconstruction images of day 9 neural spheroids derived from WTC iPSCs-SOX10GFP reporter line. The neural spheroid was immunostained with N-CADHERIN (red) and GFP (green). All organoids were counterstained with Hoechst 33342 (blue). Scale bar = 200 μm.

(D) Whole-mount staining for SOX2 (green) of oligodendrocyte brain organoids at day 21 of in vitro differentiation. Organoids were counterstained with Hoechst 33342 (blue). White arrow indicates a neural rosette. Scale bar = 200 μm.