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. 2023 Nov 16;4(4):102725. doi: 10.1016/j.xpro.2023.102725

Figure 7.

Figure 7

Characterization of oligodendrocyte brain organoids

(A) Bright-field images showing the developmental stages of oligodendrocytes brain organoids overtime in vitro. Scale bar = 400 μm.

(B) Analysis of immunostaining of organoid sections at day 140 showing expression of astrocyte marker GFAP (gold). The section was counterstained with Hoechst 33342 (cyan). Scale bar = 200 μm.

(C) Immunostaining of organoid sections on day 140 showing expression of the mature myelinating oligodendrocyte marker MBP (gold). The section was counterstained with Hoechst 33342 (cyan). Scale bar = 200 μm.

(D) Schematic diagram showing the different morphologies of MBP positive cells across organoid developmental stages.

(E) Examples of organoids sections at day 140 double immune-stained for astrocyte marker GFAP (gold) and the mature myelinating oligodendrocyte marker MBP (pink). All sections were counterstained with Hoechst 33342 (cyan). Scale bar = 25 μm.

(F) Immunostaining of organoids sections on day 120 showing expression of the pan-oligodendroglia marker OLIG2 (pink). The sections were counterstained with Hoechst 33342 (cyan). Scale bar = 200 μm. Zoomed images scale bar = 20 μm.