Neural induction medium (Olig1)

No.	Medium components	Concentration/Stock	100 mL volume
1	DMEM F12	10× 500 mL	47.45 mL
2	N2 Supplement 5 mL (100×)	Supplemented at 1%	1 mL
3	B 27 Supplement 10 mL	Supplemented at 2%	2 mL
4	MEM Non-Essential Amino Acids Solution (100×)	Supplemented at 1%	1 mL
5	Penicillin-Streptomycin (10,000 U/mL)	Supplemented at 1%	1 mL
6	2-Mercaptoethanol k(1000×)	Supplemented at 0.1%	0.1 mL