Expansion medium (Olig2)

No.	Medium components	Concentration/Stock	100 mL volume
1	DMEM F12 Nutrient mix	10× 500 mL	95.44 mL
2	B27 without vitamin A	Supplemented at 2%	2 mL
3	N2	Supplemented at 1%	1 mL
4	MEM Non-Essential Amino Acids Solution (100×)	Supplemented at 1%	1 mL
5	Penicillin-Streptomycin (10,000 U/mL)	Supplemented at 1%	1 mL
6	10 ng/mL PDGF-AA	50 μg/mL	20 μL
7	10 ng/mL IGF	100 μg/mL	10 μL
8	10 ng/mL NT3	50 μg/mL	20 μL
9	60 ng/mL T3	600 μg/mL	10 μL
10	100 ng/mL biotin	1 mg/mL	10 μL
11	10 μM cAMP	100 mM	1 μL
12	10 ng/mL HGF	50 μg/mL	20 μL
13	2-Mercaptoethanol 50 mL (1000×)	Supplemented at 0.1%	0.035 mL