No. | Olig3 medium components | Concentration/Stock | 100 mL volume |
---|---|---|---|
1 | DMEM F12 Nutrient mix 10× 500 mL | 1:1 ratio of DMEM/F12 and Neurobasal medium | 47.67 mL |
Neurobasal Medium | 47.67 mL | ||
2 | B27 without vitamin A | Supplemented at 1% | 1 mL |
3 | N2 | Supplemented at 0.5% | 0.5 mL |
4 | MEM Non-Essential Amino Acids | Supplemented at 1% | 1 mL |
5 | GlutaMAX | Supplemented at 1% | 1 mL |
6 | Pen/Strep | Supplemented at 1% | 1 mL |
7 | 10 ng/mL PDGF-AA | 50 μg/mL | 20 μL |
8 | 10 ng/mL IGF | 100 μg/mL | 10 μL |
9 | 10 ng/mL NT3 | 50 μg/mL | 20 μL |
10 | 60 ng/mL T3 | 600 μg/mL | 10 μL |
11 | 100 ng/mL biotin | 1 mg/mL | 10 μL |
12 | 10 μM cAMP | 100 mM | 1 μL |
13 | 10 ng/mL HGF | 50 μg/mL | 20 μL |
14 | 2-Mercaptoethanol (1000×) | 0.35 μL/mL | 35 μL |
15 | Insulin | 9.5–11.5 mg/mL | 25 μL |