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. 2023 Nov 16;4(4):102725. doi: 10.1016/j.xpro.2023.102725

Differentiation and maturation medium (Olig3)

No. Olig3 medium components Concentration/Stock 100 mL volume
1 DMEM F12 Nutrient mix 10× 500 mL 1:1 ratio of DMEM/F12 and Neurobasal medium 47.67 mL
Neurobasal Medium 47.67 mL
2 B27 without vitamin A Supplemented at 1% 1 mL
3 N2 Supplemented at 0.5% 0.5 mL
4 MEM Non-Essential Amino Acids Supplemented at 1% 1 mL
5 GlutaMAX Supplemented at 1% 1 mL
6 Pen/Strep Supplemented at 1% 1 mL
7 10 ng/mL PDGF-AA 50 μg/mL 20 μL
8 10 ng/mL IGF 100 μg/mL 10 μL
9 10 ng/mL NT3 50 μg/mL 20 μL
10 60 ng/mL T3 600 μg/mL 10 μL
11 100 ng/mL biotin 1 mg/mL 10 μL
12 10 μM cAMP 100 mM 1 μL
13 10 ng/mL HGF 50 μg/mL 20 μL
14 2-Mercaptoethanol (1000×) 0.35 μL/mL 35 μL
15 Insulin 9.5–11.5 mg/mL 25 μL