REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SOX1 (1:150)	R&D Systems	AF2018
SOX2 (ICC [1:300], whole mount [1:150])	Cell Signaling Technology	D9B8N
SOX10 (ICC [1:300], whole mount [1:150])	Cell Signaling Technology	89356
OLIG2 (1:300)	In Vitro Technologies	RDSAF2418SP
GFAP (1:300)	Thermo Fisher Scientific	71-1500
MBP (1:300)	Cell Signaling Technology	78896
NANOG (1:300)	Cell Signaling Technology	4893
N-CADHERIN (ICC [1:300], whole mount [1:150])	Cell Signaling Technology	14215
OCT4 (1:300)	Bio-Rad	mca5683
CTIP2 (1:300)	Abcam	ab18465
SATB2 (1:300)	Abcam	Ab51502
ZO-1 (1:150)	Thermo Fisher Scientific	33-9100
HOXC9 (1:150)	DSHB	5B5-2-s
Hoechst (1:1,000)	Abcam	AB228551-5ML
Chemicals, peptides, and recombinant proteins
Matrigel hESC-qualified matrix	Corning	354277
Neurobasal medium	Invitrogen	21103049
DMEM-F12	Thermo Fisher Scientific	11320033
GlutaMAX supplement	Thermo Fisher Scientific	35050061
N-2 supplement	Thermo Fisher Scientific	17502001
B27 supplement without vitamin A	Invitrogen	12587-010
MEM non-essential amino acids solution (100×)	Thermo Fisher Scientific	11140050
Penicillin/Streptomycin (P/S)	Invitrogen	15140122
T3	Sigma-Aldrich Pty Ltd.	T2877-250MG
NT3	Capsugel	450-03-100UG
Human IGF-I (1 mg)	Capsugel	100-11-1000
Human HGF	Capsugel	100-39H-100
FGF	In Vitro Technologies Pty Ltd.	RDS233FB01M
Biotin	Sigma-Aldrich Pty Ltd.	B4639-1G
PDGF-AA	Capsugel	100-13A-100UG
CAMP	Sigma	D0627
SB-431542	Merck	US1616464-5MG
LDN193189	Sigma	SML0559-5MG
2-Mercaptoethanol	Thermo Fisher Scientific	21985023
Insulin	Sigma-Aldrich Pty Ltd.	I9278-5ML
ReLeSR	STEMCELL Technologies	100-0484
PBS	Thermo Fisher Scientific	20012027
HBSS	Thermo Fisher Scientific	88284
Dispase	Thermo Fisher Scientific	17105041
mTeSR Plus	STEMCELL Technologies	100-0276
Software and Algorithms
GraphPad Prism		https://www.graphpad.com/features
Adobe Illustrator		https://www.adobe.com/products/illustrator.html
Fiji		https://fiji.sc/
BeeNet v1		https://honeycombbio.zendesk.com/hc/en-us/articles/4408704149147-BeeNet-v1-1-X-Installation-Video
Seurat software		https://satijalab.org/seurat/
Experimental models: Cell lines
Human ESCs	G22, Mel-1	A13777
Human iPSCs	WTC, EU79, HBSL3i	EU798 and HBSL3i were generated in-house in the Wolvetang lab; WTC is a kind gift of Bruce Conklin
Other
Ultra-low attachment 24-well plate	In Vitro Technologies Pty Ltd.	COR3473
U-bottom ultra-low attachment plates, 96 well	Sigma	CLS7007-24EA
Ultra-low attachment plates, 6 well	Sigma	CLS7007-24EA
6-Well plate	Sigma-Aldrich Pty Ltd.	CLS3516-50EA
Scissors and forceps	N/A