Table 2. Chronic stress protocol and timing.

Day	Stress treatment
1	1hr restraint, alone
2	2 hr in new cage with wet bedding
3	Alone, Forced swim test, 5mins
4	Tail pinches in restrainer, 20 mins
5	Cold water swim test, 5mis
6	Crowding, overnight
7	Isolation, overnight
8	1hr restraint, alone
9	2 hr in new cage with wet bedding
10	Alone, Forced swim test, 5mins
11	Tail pinches in restrainer, 20 mins
12	Cold water swim test, 5mis
13	Crowding, overnight
14	Isolation, overnight
15	1hr restraint, alone
16	2 hr in new cage with wet bedding
17	Alone, Forced swim test, 5mins
18	Tail pinches in restrainer, 20 mins
19	Cold water swim test, 5mis
20	Crowding, overnight
21	Isolation, overnight
22	1hr restraint, alone
23	2 hr in new cage with wet bedding
24	Alone, Forced swim test, 5mins
25	Tail pinches in restrainer, 20 mins
26	Cold water swim test, 5mis
27	Crowding, overnight
28	Isolation, overnight