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Inhibition by DFO of 4-POBN–ethanol spin trapping of ·OH in wild-type and SOD-deficient E. coli. The EPR spectrum was obtained 10 min after addition of 100 μM H2O2 to Chelex-treated HBSS containing 100 μM DTPA, 10 mM 4-POBN, 170 mM ethanol, and wild-type E. coli (2.5 × 109/ml) (A) pretreated for 50 min with 2 mM DFO (B) or containing SOD-deficient E. coli (2.5 × 109/ml) (C) pretreated for 50 min with 2 mM DFO (D). DSFL, DFO.
