Table 6.
Potential biomarkers for liquid biopsy in breast cancer bone metastases
| Biomarkers | Category | Techniques used | Refs. |
|---|---|---|---|
| RANKL | Protein | ELISA | [88] |
| SCUBE2 | Protein | ELISA | [28] |
| P1NP, CTX, 1-CTP | Protein | Immunological analysis | [96] |
| uNTx | Protein | ELISA | [147] |
|
IBSP Exosomal miRNA-19a |
RNA, protein |
ELISA (IBSP) RT-qPCR (Exosomal miRNA-19a) |
[98] |
| miRNA-30b-5p | RNA | RT-qPCR | [99] |
| miR-378 and miR-16 | RNA | RT-qPCR | [102] |
| HSP90AA1, SPP1, IL3, VEGFA, PTK2 | Gene panel | RT-qPCR | [120] |
| CTC | Cell | Immunomagnetic sorting | [136, 137] |
| cOC | Cell | Flow cytometry and immunofluorescence staining | [140] |