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. 2023 Dec 2;56:63. doi: 10.1186/s40659-023-00476-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The main models to mantain the interaction between gastrointestinal cancers PDOs and immune cells. A Model based in the use of ALI-PDOs that preserve the immune cells for almost 60 days. B Co-culture interaction between PDOs and T-cells derived form same patient (autologous) with the aim to generate tumor-responsive as a cancer treatment. C Co-culture interaction based on PDOs and PBMC derived from healthy controls (allogenic) with the aim to generate cytotoxicity activity against to the tumor cells from CD8 T-cells. The figure was created with Biorender