FIG. 2.
Expression of algK in E. coli. (A) Partial restriction map of P. aeruginosa FRD1 DNA in pMF100, showing a 3.8-kb EcoRI fragment containing algK and alg44 that was used in this study for expression in E. coli under control of the T7 promoter (PT7). (B) Autoradiogram of [35S]methionine-labeled proteins expressed in E. coli JM109(DE3) from the vector pBluescript II KS(−) (lane 1) or the clone pMF100 (lane 2) in the presence of IPTG and rifampin. Sizes of molecular weight markers (lane 3), in thousands, are shown. The positions of the AlgK and Alg44 proteins expressed from pMF100 are indicated with arrows.