Table 1. Primers used in the present study.
Target gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplification length (bp) | Reference |
---|---|---|---|---|
COI | 5′- CAGCTTTTGTAGAGGGCG - 3′ | 5′- CCAAATTACACCAGTCAGCC - 3′ | 722 | this study |
5′- GATCCGGGATAGTAGGTAC - 3′ | 5′- CMGGATGACCAAARAACC - 3′ | 645 | this study | |
5′- ATGGCYGTCGATTTAGC - 3′ | 5′- CMGGATGACCAAARAACC - 3′ | 298 | this study | |
COI | LCO 1490 | HCO 2198 | 710 | Folmer et al. (1994) |
mlCOIintF | jgHCO2198 | 313 | Leray et al. (2013) | |
5′- GGTTGAACTATHTATCCNCC - 3′ | 5′- GAAATCATYCCAAAAGC - 3′ | 338 | Sanna et al. (2013) | |
16S rRNA | 5′- GGTAGCGAAATTCCTAGCC - 3′ | 5′- AAKGGTSGAACAGACCC - 3′ | 408 | this study |
16S rRNA | 5′- TGCTCAATGCCCAAGGGGTAAAT - 3′ | 5′- AACTCAGATCACGTAGGG - 3′ | 450 | Sanna et al. (2013) |
nad3 | 5′- CCTTATGARTGYGGBTTT - 3′ | 5′- TCHATAAGYTCATARTAYARCCC - 3′ | 203 | Sanna et al. (2014) |