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. 2023 Nov 29;11:e16491. doi: 10.7717/peerj.16491

Table 3. AMOVA table using genetic distances based on haplotype frequencies of the P. nobilis populations.

Scenarios Source of variation Degrees of
freedom
Sum of squares Components of covariance (Sigma) Variation (%) P-value
Greece (Eastern Mediterranean) (16S rRNA-COI) Ionian Sea (Amvrakikos, Korinthiakos) - North Aegean Sea (Lesvos, Aggelochori, Epanomi, Chios, Vourvourou) - South Aegean Sea (Attica, Diafani, Tristomo, Astakida, Crete) Between populations 2 18.51356 0.01582342 0.52 0.28
Between samples within population 9 57.22781 0.53133279 17.46 0.01
Within samples 88 219.5786 2.49521173 82.02 0.01
Central, Western and Eastern Mediterranean Sea (16S rRNA-COI) Adriatic Sea (Venice) - Ionian Sea (Amvrakikos, Korinthiakos, Sicily East) - North Aegean Sea (Lesvos, Aggelochori, Epanomi, Chios, Vourvourou) - South Aegean Sea (Attica, Diafani, Tristomo, Astakida, Crete) - Levantine Sea (Cyprus) - Western Mediterranean (Italy, Sicily West, Corsica, Sardinia) Between populations 5 213.2511 0.8919529 25.07 0.01
Between samples within population 13 77.38444 0.2702837 7.60 0.01
Within samples 275 658.6637 2.3951407 67.33 0.01
Mediterranean Sea (COI) Adriatic Sea (Venice) -
Ionian Sea (Amvrakikos, Korinthiakos, Sicily East) -
North Aegean Sea (Lesvos, Aggelochori, Epanomi, Chios, Vourvourou) -
South Aegean Sea (Attica, Diafani, Tristomo, Astakida, Crete) - Levantine Sea (Cyprus) -
Western Mediterranean (Bizerta Lagoon, Italy, Sicily West, Corsica, Sardinia, France, Spain) - Tunisian Plateau/Gulf of Sidra (El Ketef, Stah Jaber, Kerkennah Island, El Bibane Lagoon)
Between populations 6 32.72616 0.09382413 9.56 0.01
Between samples within population 19 20.06477 0.01168851 1.19 0.06
Within samples 423 370.5275 0.87595168 89.26 0.01