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. 2023 Nov 28;18:7065–7077. doi: 10.2147/IJN.S438229

Figure 1.

Figure 1

Pericytes declined in age-related bone resorption and bone resorption caused by estrogen deficiency (A) Representative immunostaining images of femurs from OVX and female WT mice. NG2+ (red) pericytes, endomucin+ (green) endothelial cell, and nuclear (blue) DAPI staining. Scale bar, 50 μm. (B) Quantification of NG2+ pericyte number in subepiphyseal zone of femurs from OVX and female WT mice (n = 6). (C) Representative TRAP staining images of femurs from OVX and female WT mice. Scale bar, 100 μm (lower panels); 500 μm (upper panels). (D) Quantification of osteoclast surface per bone surface (Oc.S/BS) and osteoclast number per bone perimeter (Oc.N/B.Pm) in subepiphyseal zone of femurs from OVX and female WT mice (n = 6). (E) Analysis of the Pearson correlation between NG2+ cell number and Oc.N/B.Pm in femurs from female wild-type mice of different ages (6, 12 and 18 months). Data are presented as the mean ± S.E.M. P-values < 0.05 were considered statistically significant, as follows: ***P < 0.001; ****P < 0.0001.