Figure 1. TNF-induced Jagged1 expression is mainly dependent on RBP-J.
A) Immunoblot analysis of Jagged1 expression in the WT control (Ctrl) and RbpjΔM/ΔM BMMs treated with TNFα for the indicated time periods. p38 was blotted as the loading control. B, C, D) Quantitative real-time PCR (qPCR) analysis of mRNA expression of the indicated genes after TNFα stimulation for 0h, 4h, and 24h in Ctrl and RbpjΔM/ΔM BMMs (B, C) or RANKL stimulation for 0h, 6h and 24h in the WT control cells (D), displayed relative to % GAPDH. Data in A, B and D are representative of three independent experiments. Data are mean ± SD. *p < 0.05.
